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Methods and Common Knowledge of Ordinary Culture Medium
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sterile diluent inje, sterile ziplock bags, |
| Specifications |
500000 |
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Ordinary media in microbiology: a kind of artificial preparation, suitable for the growth and reproduction of microorganisms or the production of metabolites, or for other mixed nutrients.
Characteristics: any culture medium should have the six nutrient elements needed by microorganisms and should be proportioned appropriately. Once prepared, it must be sterilized immediately.
Uses: promote the growth of microorganisms; accumulate metabolites; isolate microbial species; identify microbial species; count microbial cells; preserve microbial species; prepare microbial products.
General configuration steps of ordinary culture medium
1. Mix ingredients
Formula conversion ¡ú add a small amount of water (distilled water, natural water) into the container ¡ú weigh all kinds of drugs according to the formula (add in order) ¡ú add enough water (one medicine and one spoon, and immediately cover the bottle after taking the medicine).
2. dissolution
Starch dissolving: a small amount of cold water is mixed into paste
Heat and dissolve, especially the medium with agar must be boiled. The melting temperature of agar is 95-97 ℃ and it needs to be stirred while heating to prevent burning.
3. Adjust PH
Adjust the medium to the required value with 1n hydrochloric acid or 1n NaOH.
4. filtration
Filter paper or cotton. (sometimes it can be omitted)
5. sub package
Generally, the ordinary culture media are placed in a triangular flask or test tube for sterilization.
6. Packing
After repacking, plug the cotton plug, and wrap the cotton plug with kraft paper to prevent water from entering and wetting the cotton plug during sterilization.
7. sterilization
Carry out high-pressure steam sterilization according to the temperature and pressure required on the formula. If the sterilization temperature is too high, the nutrients will be destroyed and cultured. The sugar and amino acid in the ordinary culture medium will darken the colour of the medium.
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| Company: |
Elex Biological Products (Shanghai) Co., Ltd.
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| Contact: |
elex bio |
| Address: |
Room E121, No. 2800, Wanyuan Road, Minhang District, Shanghai City, P.R. China |
| Postcode: |
201100 |
| Tel: |
021-59868187 |
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